Size exclusion chromatography/multi-angle light scattering (SEC/MALS) is a technique used to determine the size, shape, and molecular weight of macromolecules such as proteins, polymers, and nanoparticles. In SEC/MALS, samples are separated by size using size exclusion chromatography, and then analyzed by multi-angle light scattering, which measures the scattering of light by the sample at different angles. The resulting data allows for the calculation of molecular weight, size, and other structural information of the macromolecule. SEC/MALS is widely used in fields such as biopharmaceuticals, polymer chemistry, and material science.
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