Ultimate Nano-HPLC system setup is used for LC-MS proteomics workflows. It can be coupled to Iontrap mass spectrometer or MALDI-TOF via a fraction collector (Bruker Proteineer fc II). System contains …a Method uses 25 cm Acclaim Pepmap C18 separation column with 3 µm particle size

HPLC instrument

UHPLC

Column oven temp ambient to 75 °C

loading pump 50- 2500 µl

Nano LC 50-1000 nl/min

Cap LC 0,5-10 ml/min

Micro LC 5-50 µl/min

Pre-consentrate trap column

max pressure 800 bar

RS autosampler

Fraction collecto

-          r-Bruker Proteineer fc II

Programs

-          Chromeleon

Computer

-          WIN7