Dionex Ultimate 3000 HPLC consists of LPG-3400 SD Pump (gradient pump and degasser), WPS-3000 TSL analytical autosampler, TCC-3000SD Column Thermostat, VWD-3400RS Detector, and Refractomax 521 detector.

HPLC instrument

• Low-Pressure Mixing Gradient Pump. Supports high-speed and high-resolution applications as well as standard HPLC workflows.
• Settable flows of 0.001-10 mL/min and pressures up to 620 bar, 400 µL mixer, 679 µL GDV, 4 solvent channels with integrated vacuum degasser.
• Provides high precision, excellent linearity, and optimal application support up to 620 bar.
•  Analytical scale split-loop sampler w/ sample thermostatting (4-45 °C), injection volume 1-100 μL (field-upgradable to 500 μL)
• 3 segments for vial racks/wellplates
• 3 x 48 pos. sample racks
• Thermostatted Column Compartment SD with switching valve options; 5-80 °C, forced air mode, optional passive pre-heating, holds up to 6 columns of 300 mm max. length, field-upgradeable w/ up to 2 valves
• Refractomax 521detector offers non-destructive, universal detection for isocratic separations, ideal for the use with substances with no or limited UV/Vis activity. Extended flow rate range from 1 to 10 mL/min and the operating range from 1.00 to 1.75 RIU for a wide range of application.
• VWD 3400RS detector supports a wide range of applications, for superior detection of trace analytes with low noise and drift, and a wide selection of flow cells. Up to 200 Hz data rate, multi-wavelength mode for max. 4 channels (max. 5 Hz each) by sequential acquisition, UV and Vis lamp for enhanced sensitivity at higher wavelengths, 190-900 nm.
• Flow cell, VWD: SST, 11 µL Volume, 10 mm Pathlength

Program(s)

• Chromeleon 7.3