PCR

Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify a specific DNA sequence. In PCR, the targeted DNA sequence is repeatedly copied in vitro using a thermostable DNA polymerase enzyme, oligonucleotide primers, and nucleotides. Thermal cycling is used to denature the DNA, anneal the primers, and activate the DNA polymerase, which then extends the primers and synthesizes the complementary strands. PCR is widely used for research, genetic testing, and medical diagnostics.